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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Prevalence, Risk Factors and Genotyping of Cryptosporidium spp.from Feces of Dairy Cows in Saraburi, Kanchanaburi and Nakhon Pathom Provinces) ผู้เขียน:Tawin Inprankaew, ดร.พิพัฒน์ อรุณวิภาส, รองศาสตราจารย์, นางนงนุช ภิญโญภานุวัฒน์, นายวิษณุวัฒน์ ฉิมน้อย, ดร.สถาพร จิตตปาลพงศ์, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe aims of this study were to determine the prevalence, risk factors and genotype of Cryptosporidium spp. in dairy cows raised in Saraburi, Kanchanaburi and Nakhon Pathom Provinces. A total of 400 fecal samples were randomly collected and examined for Cryptosporidium spp. by the DMSO-modified acid-fast stain technique and polymerese chain reaction restriction fragment length polymorphism (PCR-RFLP). The prevalence of Cryptosporidium infection in dairy cows was 0.5% (2/ 400) by acid-fast staining and 1.3% (5/400) by PCR-RFLP. Age was the only significant risk factor for infection in dairy cows (p<0.05). All PCR positive samples were C.parvum (bovine genotype). The result indicated a potential risk of cryptosporidiosis transmission to humans, particularly to workers in close contact with cows. |